Application of Tissue Culture Room in Artificial Seed Breeding

The material with normal development ability is obtained through the method of plant tissue culture, and the material capable of germinating into plant larvae under suitable conditions is called artificial seed. The concept of "artificially developing material" in the concept of artificial seed usually refers to the embryoid body (currently there are foreign shoots, apical shoots, and axillary buds) and it can be generated in three ways: (1) from Differentiated explants are produced directly; (2) produced by callus; (3) produced by suspension cell culture. The basic requirements for embryoid bodies are: 1 seedlings emerge quickly; 2 shoots and shoots grow almost simultaneously without callus formation; 3 high degree of synchronization; 4 strong vigor; 5 seedling formation and growth should be normal . In the cultivation process, the tissue culture chamber is an indispensable experimental instrument and is very useful for the synthesis of artificial seeds.

The explants used in plant tissue culture are generally highly differentiated cells that do not divide and multiply in the plant body, but perform some function until death. When these cells are cultured on the culture medium of the tissue culture chamber, they will be differentiated from their original state, and they will be transformed into cells in the raw state and will be divided to produce callus. This process is called dedifferentiation. This transformation will produce a series of changes in the morphological structure and physiology of the cells. The results of tissue culture studies indicate that dedifferentiation of differentiated cells requires two conditions, namely trauma and exogenous hormones.

For plant tissue culture, lighting conditions are very important, including light intensity, time, and wavelength. However, in the callus induction stage, dark culture is often required, and in the process of differentiation and regeneration, there must be light. The dark culture of callus induction phase is conducive to the dedifferentiation of cells to produce callus. If it is easy to differentiate and produce vascular and other tissues under light conditions, it is not conducive to generate a large number of callus. Therefore, the control of these conditions in the tissue culture room must be very precise, so as to better promote the success of the organization.

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